General Cell Based Assays
Eurofins Discoverx PathHunter eXpress LTB4R CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0292E2CP0M)
The PathHunter LTB4R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. leukotriene B4) based activation of LTB4R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged LTB4R and EA-tagged β-Arrestin-2. Activation of LTB4R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CRHR2 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0251E2CP0L)
The PathHunter CRHR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CRH, sauvagine) based activation of CRHR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CRHR2 and EA-tagged β-Arrestin-2. Activation of CRHR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Biotium CF488A and PI Apoptosis Assay Kit
Phosphatidylserine (PS) is translocated from the inner to the outer surface of the cell for phagocytic cell recognition. The human anticoagulant, Annexin V, is a 35 kDa Ca2 -dependent phospholipid protein with a high affinity for PS. Annexin V labeled with CF488A (Abs/Em= 490/515 nm) can identify apoptotic cells in green by binding to PS exposed on the outer leaflet. Our CF488A dye is superior to fluorescein/FITC as it is not affected by pH and has much better photostability. Propidium iodide (PI) is impermeant to live cells or early apoptotic cells, but stains necrotic cells and late apoptotic cells with red fluorescence (Abs/Em with DNA = 535/617 nm). Both PI and Annexin V-CF488A can be excited using the 488 nm line of a flow cytometer. The kit has reagents sufficent for 100 assays.
Eurofins Discoverx PathHunter eXpress TORC1 Nuclear Translocation Assay, 2pl Kit. (93-0833E3CP19M)
The PathHunter eXpress TORC1 Nuclear Translocation Assay provides an easy-to-use cell based assay to study drug candidates affecting the CRTC/CREB pathway. This kit assesses ligand (e.g. forskolin) based pathway activation via detection of resulting TORC1 (CRTC1) nuclear translocation. This product uses EFC technology. The included cell line overexpresses ProLabel-tagged TORC1 (CRTC1) and nuclear localized EA. Upon pathway activation, induced nuclear translocation of TORC1 leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Grainger G0365 Toolboard Accessory Kit 1 in
The Wall Control Toolboard Accessory Kit is a set of 29 metal hooks and bins that attach to the Wall Control galvanized steel pegboard to organize tools and supplies. The kit includes 12 small steel bins, 12 large steel bins, and 5 steel hooks. The bins have side handles for easy removal and can hold small parts, craft supplies, office items, and other accessories. The hooks allow hanging lightweight tools. All pieces have pegboard hooks to securely mount to the Wall Control board. This kit is a convenient way to add storage and organization to the pegboard.
Eurofins Discoverx PathHunter eXpress TrkB Functional Assay, 10-Plate Kit. (93-0463E3CP16L)
The PathHunter eXpress TrkB Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated TrkB dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged TrkB and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Indigo Biosciences Human Oxytocin Receptor (OXTR) All-inclusive Cell-Based Luciferase Reporter Assay Kit, 384-well assay plate format
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human Oxytocin Receptor (OXTR) reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against OXTR. Kit includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed assay protocol. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request). This kit allows researchers to achieve superior sensitivity, clear, reproducible results, and eliminate weeks of cell culture work.
Cayman Chemical TGR5 (GP-BAR1) Reporter Assay Kit, 1 ea
Cayman's TGR5 (GP-BAR1) Reporter Assay Kit consists of a 96-well plate coated with a transfection complex containing DNA constructs for expressing TGR5 and a cAMP response element regulated secreted alkaline phosphatase (SEAP) reporter (TGR5 Reverse Transfection Strip Plate).; Cells grown on the transfection complex will express TGR5 at the cell surface within 24 hours.; Binding of agonists to TGR5 initiates a signal transduction cascade through the GαS and adenylate cyclase pathway resulting in the expression of SEAP which is secreted into the cell culture medium.; Aliquots of culture medium are collected at 6-8 hours after stimulation, and SEAP activity is measured following the addition of a luminescence-based alkaline phosphatase substrate provided in the kit.; The kit provides sufficient reagent to measure SEAP activity at three time points using the three included white assay plates.
Eurofins Discoverx PathHunter eXpress HTR2C (VNV) U2OS β-Arrestin GPCR Assay, 10pl Kit. (93-0938E3CP19L)
The PathHunter HTR2C(VNV) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. serotonin) based activation of HTR2C(VNV) GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HTR2C(VNV) and EA-tagged β-Arrestin-2. Activation of HTR2C(VNV) stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress CXCR4 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0081E2CP2M)
The cAMP Hunter CXCR4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL12/SDF-1α) based activation of CXCR4 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CXCR4, a naturally Gi/Go coupled GPCR. Upon activation of CXCR4, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human MMP-8 ASY
The R-PLEX Human MMP-8 Assay Kit from Meso Scale Discovery is a multiplex immunoassay that measures MMP-8 protein levels in human serum plasma saliva and cell culture supernatants The assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and requires low sample volume The kit includes a 96-well plate coated with capture antibody detection antibody read buffer wash buffer blocker solutions calibrator and controls The assay has a typical sensitivity of 0 08 ng/mL and an upper limit of quantification of 1500 ng/mL
TARGETMOL CHEMICALS INC P38 MAPK INHIBITOR 5MG
Also available in 1 mg 10 mg 25 mg 50 mg 100 mg 500 mg and bulk. Please contact Fisher for quotes. p38 MAPK inhibitor is a potent inhibitor of p38 MAP kinase (IC50 = 35 nM). It inhibits senescence induced by the oncogene RAS. purity: 99%
Eurofins Discoverx PathHunter eXpress mADCYAP1R1 (PAC1) CHO-K1 β-Arrestin GPCR Assay, 2pl Kit. (93-0594E2MCP0M)
The PathHunter mADCYAP1R1 (PAC1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. PACAP) based activation of mADCYAP1R1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mADCYAP1R1 and EA-tagged β-Arrestin-2. Activation of mADCYAP1R1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human SDF-1 alpha (CXCR4) β-Arrestin Cell-Based Assay Kit (C2C12)
The PathHunter Human SDF-1 alpha (CXCR4) β-Arrestin Stable Cell Line Assay (C2C12) contains a stable clonal cell line used to measure CXCR4 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Discovery Life Sciences Gentest Cryo White Rabbit Hep
Gentest Cryopreserved New Zealand White Rabbit Hepatocytes Female plateable